Fig. 3.

Plate assays demonstrating that decoy oligonucleotides can influence antibiotic production in S. coelicolor. Filter discs were saturated with solutions of decoys or, as control, buffer and applied to a lawn of S. coelicolor M145 overlaid with SNA medium. As the bacteria continued to grow, enhanced antibiotic production was recognized by early accumulation of the pigmented antibiotics around the disks (evident 48 h after the addition of the decoys). Negative controls (buffer alone or a “scrambled” version of decoy A24.5) did not show precocious production.