Table 1.
Name | Sequence |
---|---|
Decoy oligonucleotides | |
A24.1 | 5′-P-atatcactctcgatgtcggcgttttcgccgacatcgagagtgatatagttttct-3′ |
A24.2 | 5′-P-atatcaggaatgccagatgcgttttcgcatctggcattcctgatatagttttct-3′ |
A24.3 | 5′-P-atactgcctctcggtaagcgttttcgcttaccgagaggcagtatagttttct- 3′ |
A24.4 | 5′-P-atatgcagctcgctgcacgcgttttcgcgtgcagcgagctgcatatagttttct-3′ |
A24.5 | 5′-P-ataaatctgttgagtagggcgttttcgccctactcaacagatttatagttttct-3′ |
A24.5 scrambled | 5′-P-atagcaatttagatggtggcgttttcgccaccatctaaattgctatagttttct-3′ |
For quantitative PCR for decoy copy number | |
A24.1 copy f | 5′-tcgccgacatcgagagtgatat-3′ |
A24.1 copy r | 5′-aaacgccgacatcgagagtga-3′ |
For PCR deletion of SCO5812 | |
5812KOf | 5′-cgcccggcgatcgcggcggcgtacgctagggacgccatggctacgtgtaggctggagctgcttc-3′ |
5812KOr | 5′-tcggcgggtggcacatacctgcgagcgcgagcgggaccgtcagctagcattccggggatccgtcgacc-3′ |
5812Testf | 5′-tcccgcttttgggaccggggtgt-3′ |
5812Testr | 5′-atcaaacgttggtgcggctcgcg-3′ |
For PCR cloning of SCO5812 for complementation | |
5812exf | 5′-aaagatcgaacatatgccgtacgaaccacctacgcac-3′ |
5812exr | 5′-ttacgatcaagctttcagaaatcgaatccgagctgaccc-3′ |
The cis-regulatory sequences in each of the decoy oligonucleotides are boldface.