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Relationship between the 15N chemical shifts and the wavelengths of maximum visible absorption in the 13-cis,15-anti retinylidenes of bacteriorhodopsin (filled circles) and a series of 13-cis,15-anti retinylidene halides (open circles). The dashed line is a linear fit to the halide series (28). We identify our major, persistent L (L185) with the major, late-L (“L2”) component in time-resolved optical spectra (22).
