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Chromatography analysis of the products of Ars reactions. (A and B) HPLC chromatograms of lipid extracts prepared from E. coli harboring pETDuet–ArsAD and pACYC–ArsB (A) or pETDuet–ArsAD and pCDF–ArsC (B). (C and D) Negative extracted ion LC-APCIMS chromatograms of in vitro reactions containing ArsA, ArsB, and ArsD (C) and ArsA, ArsC, and ArsD (D).
