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. Author manuscript; available in PMC: 2008 Feb 13.
Published in final edited form as: J Biol Chem. 2003 Nov 10;279(8):6783–6793. doi: 10.1074/jbc.M309393200

Fig. 5. Colocalization of methyl-binding proteins with nucleolin in HepG2 cells.

Fig. 5

Co-localization studies were performed with antibodies specific for methyl-binding proteins raised in rabbit (except MBD2, which was raised in sheep) and monoclonal antibody (C23) against nucleolin, a nucleolus-specific protein. MBDs were recognized by fluorescein isothiocyanate-conjugated secondary antibody (green fluorescence), and nucleolin was recognized by TRITC-conjugated secondary antibody (red fluorescence). The yellow signal indicates co-localization of MBDs with nucleolin.