Table 1.
Derivative | [Pep] w/w | Number of peaks | % Area at 1625 cm−1 | % Area at 1685 cm−1 | Total % area | Estimated number of carbonyls | r2 |
---|---|---|---|---|---|---|---|
Cys-Ala | 10% | 5 | 73 | 14 | 87 | 7 | 0.998 |
Lan-β-M | 10% | 4 | 73 | 9 | 82 | 7 | 0.997 |
This estimation was done on the spectra recorded for the highest concentration of peptide to reduce the contribution of the unstructured spectra arising from nonassembled peptides. The decomposition was performed using simple Gaussian curves (see Fig. 2).