Table 2.
Production of Gag antigen SIV in dividing and non-dividing CEMx174 cells.
| Virus | MTT1 +aphid1day/+aphid 3 days (OD) | Viability index (fold difference) | p27 ng/ml2 -aphid.3 days | p27 ng/ml2 +aphid.3 days (x3)3 |
| SIVmac239 | 0.327/0.106 | 3 | 28 | 17 |
| SIVmac239T | 0.302/0.105 | 2.9 | 32 | 41 |
| SIVmac18 | 0.334/0.104 | 3.2 | 10 | 12 |
| SIVmac18T | 0.386/0.117 | 3.7 | 19 | 41 |
1MTT assay is described in Methods
2Amount of Gag p27 antigen in supernatants determined by ELISA described in Methods
3amount was adjusted in according to results of MTT assay.