Table 1.
Overlap of the ER stress and osmotic-stress transcriptional responses*
| Accession No. of Proteina | e-valueb | Accession No. of Clonec | PEGd | p-valuee | TUNd | p-valuee | AZCd | p-valuee | |
| Genes induced by all of the treatments | |||||||||
| ATAF2 protein | BAC43493 | 8.E-31 | AW459852 | 3.93 | 0.002 | 4.33 | 0.031 | 7.19 | 0.002 |
| NAM protein | ABE79286 | 1.E-33 | AW459732 | 2.81 | 0.008 | 3.31 | 0.019 | 5.27 | 0.001 |
| N-rich protein | CAI44933 | 4.E-07 | AI973541 | 2.76 | 0.005 | 3.39 | 0.009 | 4.33 | 0.012 |
| N-rich protein | CAI44933 | 2.E-76 | AW184865 | 1.93 | 0.008 | 2.14 | 0.012 | 2.42 | 0.003 |
| ubiquitin-associated (UBA) protein | XP_466502 | 4.E-38 | AW508375 | 3.05 | 0.000 | 3.12 | 0.024 | 2.59 | 0.014 |
| eukaryotic translation initiation factor 5 | P48724 | 2.E-65 | AW472364 | 3.56 | 0.002 | 1.54 | 0.028 | 1.84 | 0.015 |
| glutathione S-transferase | AAC18566 | 2.E-46 | AW472161 | 3.83 | 0.003 | 3.19 | 0.002 | 2.75 | 0.002 |
| glutathione S-transferase | AAG34800 | 1.E-48 | AW397276 | 2.22 | 0.002 | 2.28 | 0.000 | 1.48 | 0.009 |
| unknown | AW186110 | 2.58 | 0.005 | 3.17 | 0.003 | 9.56 | 0.000 | ||
| unknown | AW508115 | 1.74 | 0.001 | 3.08 | 0.006 | 19.56 | 0.000 | ||
| Genes repressed by all the treatments | |||||||||
| oxygen-evolving enhancer protein 1 | P26320 | 3.E-39 | AI941034 | -3.27 | 0.000 | -1.82 | 0.009 | -3.07 | 0.001 |
| thylakoid membrane phosphoprotein | NP_566086 | 1.E-21 | AI960735 | -1.93 | 0.004 | -1.65 | 0.013 | -2.03 | 0.011 |
| NADPH-protochlorophyllide oxidoreductase | BAA21089 | 4.E-78 | AW277941 | -1.76 | 0.023 | -2.08 | 0.009 | -2.16 | 0.015 |
| oxygen evolving enhancer protein 1 precursor | BAA96365 | 5.E-23 | AW101019 | -3.42 | 0.011 | -2.24 | 0.001 | -1.70 | 0.001 |
| photosystem I chlorophyll a/b-binding protein | CAA45523 | 4.E-75 | AW397435 | -9.07 | 0.001 | -4.96 | 0.009 | -7.02 | 0.000 |
| photosystem I chlorophyll a/b-binding protein | CAA45523 | 1.E-75 | AW101657 | -5.56 | 0.010 | -2.38 | 0.015 | -2.06 | 0.003 |
| chlorophyll a/b-binding protein CP24 precursor | AAD27882 | 3.E-41 | AI736217 | -7.54 | 0.000 | -5.72 | 0.003 | -10.78 | 0.001 |
| chlorophyll a/b binding protein type II | AAL29886 | 3.E-18 | AI736285 | -7.45 | 0.006 | -3.69 | 0.048 | -10.00 | 0.000 |
| LHCII type III chlorophyll a/b binding protein | AAD27877 | 9.E-85 | AW397809 | -15.20 | 0.001 | -10.48 | 0.004 | -38.20 | 0.000 |
| chlorophyll a-b binding protein | P13869 | 2.E-85 | AW471940 | -5.19 | 0.005 | -4.23 | 0.016 | -8.21 | 0.001 |
| RuBisCO small subunit 1 | CAA23736 | 7.E-53 | AW278725 | -4.74 | 0.003 | -5.01 | 0.107 | -58.93 | 0.000 |
| photosystem II type I chlorophyll a/b-binding protein | AAA50172 | 1.E-64 | AW472492 | -28.79 | 0.002 | -10.26 | 0.028 | -31.02 | 0.000 |
| oxygen-evolving enhancer protein 1 | P26320 | 8.E-64 | AW567782 | -2.11 | 0.030 | -1.65 | 0.050 | -1.58 | 0.005 |
| chlorophyll a/b-binding protein CP24 precursor | AAD27882 | 2.E-75 | AW568341 | -9.95 | 0.005 | -6.83 | 0.004 | -16.75 | 0.000 |
| putative chlorophyll a/b-binding protein precursor | XP_482572 | 2.E-73 | AW568620 | -11.01 | 0.004 | -6.02 | 0.004 | -8.13 | 0.000 |
| oxygen evolving enhancer protein 1 precursor | BAA96365 | 9.E-67 | AW568090 | -3.99 | 0.014 | -4.53 | 0.037 | -14.93 | 0.000 |
| type II chlorophyll a/b binding protein | CAA57492 | 1.E-78 | AW100823 | -3.61 | 0.000 | -2.93 | 0.024 | -9.04 | 0.001 |
| chlorophyll a/b-binding protein type I | AAQ54512 | 1.E-36 | AW100631 | -5.39 | 0.000 | -2.70 | 0.006 | -3.23 | 0.004 |
| chlorophyll a/b-binding protein type III precursor | S04125 | 2.E-43 | AI794678 | -7.95 | 0.000 | -3.36 | 0.037 | -10.81 | 0.000 |
| LHCII type III chlorophyll a/b binding protein | AAD27877 | 2.E-22 | AW508739 | -7.35 | 0.006 | -3.23 | 0.007 | -2.99 | 0.003 |
| chlorophyll a/b-binding protein CP24 precursor | AAD27882 | 8.E-72 | AW568252 | -7.94 | 0.011 | -3.40 | 0.002 | -3.53 | 0.000 |
| chlorophyll a/b-binding protein CP24 precursor | AAD27882 | 6.E-88 | AW570380 | -5.53 | 0.001 | -5.23 | 0.012 | -10.41 | 0.002 |
| photosystem I subunit × precursor | AAL32043 | 4.E-46 | AW277960 | -4.65 | 0.014 | -3.72 | 0.009 | -4.81 | 0.000 |
| oxygen-evolving enhancer protein 1 | P14226 | 4.E-71 | AW472001 | -4.74 | 0.005 | -3.13 | 0.028 | -5.54 | 0.000 |
| photosystem I psaH protein | AAQ21121 | 3.E-53 | AW471851 | -3.39 | 0.003 | -2.98 | 0.002 | -4.82 | 0.002 |
| LHCII type III chlorophyll a/b binding protein | AAD27877 | 6.E-29 | AW472547 | -9.06 | 0.001 | -3.54 | 0.019 | -1.62 | 0.004 |
| photosystem II reaction center W protein | CAA59409 | 1.E-28 | AW471847 | -2.55 | 0.015 | -1.74 | 0.024 | -1.49 | 0.002 |
| photosystem II protein | AAM61462 | 6.E-07 | AW508451 | -4.91 | 0.009 | -2.91 | 0.003 | -1.72 | 0.004 |
| photosystem I reaction center subunit III | AAD27880 | 1.E-82 | AW508794 | -5.25 | 0.002 | -4.83 | 0.030 | -33.31 | 0.000 |
| geranylgeranyl hydrogenase | AAD28640 | 9.E-48 | AW185978 | -2.18 | 0.014 | -1.89 | 0.003 | -2.37 | 0.010 |
| ultraviolet-B-repressible protein | AAS58469 | 1.E-29 | AW317705 | -5.24 | 0.003 | -4.16 | 0.011 | -10.16 | 0.000 |
| glutamine synthetase precursor | AAK43833 | 4.E-66 | AI736144 | -2.28 | 0.010 | -2.17 | 0.007 | -2.19 | 0.010 |
| myo-inositol-1-phosphate synthase | AAK72098 | 7.E-95 | AI941146 | -2.30 | 0.001 | -1.71 | 0.011 | -2.30 | 0.006 |
| UDP-glucose 4-epimerase | Q43070 | 4.E-83 | AI856802 | -3.52 | 0.001 | -2.64 | 0.001 | -3.32 | 0.007 |
| putative auxin-amidohydrolase precursor | CAG32961 | 1.E-18 | AW278733 | -4.23 | 0.001 | -3.33 | 0.007 | -8.72 | 0.000 |
| granule-bound starch synthase Ib precursor | BAC76613 | 5.E-49 | AW508018 | -1.67 | 0.004 | -2.21 | 0.031 | -2.16 | 0.006 |
| selenium binding protein | CAC67501 | 6.E-50 | AW101647 | -4.82 | 0.008 | -4.94 | 0.021 | -28.20 | 0.000 |
| ATP-dependent helicase | NP_850847 | 1.E-58 | AW570395 | -3.97 | 0.003 | -5.72 | 0.016 | -12.70 | 0.000 |
| microsomal omega-3 fatty acid desaturase | BAC87757 | 1.E-86 | AI960953 | -1.70 | 0.009 | -1.71 | 0.012 | -3.54 | 0.000 |
| granule-bound starch synthase Ib precursor | BAC76613 | 4.E-65 | AW472193 | -7.20 | 0.008 | -6.51 | 0.000 | -17.58 | 0.000 |
| carboxylic ester hydrolase | NP_177281 | 2.E-51 | AW278929 | -2.16 | 0.038 | -3.73 | 0.023 | -2.30 | 0.010 |
| cinnamoyl-CoA reductase | AAY86360 | 5.E-60 | AW508388 | -3.11 | 0.007 | -4.27 | 0.004 | -5.60 | 0.000 |
| transformer-SR ribonucleoprotein | CAA70700 | 3.E-38 | AW568037 | -3.07 | 0.011 | -2.82 | 0.000 | -7.39 | 0.000 |
| putative cinnamoyl-CoA reductase | AAT39306 | 2.E-16 | AW101559 | -2.91 | 0.004 | -3.01 | 0.005 | -1.68 | 0.010 |
| myo inositol 1-phosphate synthase | CAJ15162 | 5.E-56 | AW100674 | -4.42 | 0.000 | -4.48 | 0.003 | -5.52 | 0.001 |
| aldose 1-epimerase-like protein | NP_566594 | 3.E-66 | AW507799 | -2.36 | 0.009 | -2.74 | 0.012 | -2.26 | 0.000 |
| amino acid binding/ACT domain-containing protein | NP_565908 | 5.E-35 | AW508692 | -2.57 | 0.032 | -1.75 | 0.002 | -1.58 | 0.043 |
| cytochrome P450 monooxygenase | AAD38930 | 8.E-55 | AW507877 | -1.86 | 0.009 | -3.74 | 0.017 | -2.98 | 0.027 |
| palmitoyl-acyl carrier protein thioesterase | AAD01982 | 3.E-25 | AW568268 | -2.58 | 0.039 | -1.71 | 0.021 | -5.28 | 0.001 |
| 1-aminocyclopropane-1-carboxylate oxidase | AAX84675 | 3.E-86 | AW508290 | -6.79 | 0.010 | -5.34 | 0.020 | -11.62 | 0.000 |
| plasma membrane polypeptide | CAB61742 | 1.E-40 | AW459777 | -1.83 | 0.028 | -2.05 | 0.005 | -1.67 | 0.016 |
| acid phosphatase | CAA11075 | 8.E-20 | AI930921 | -3.69 | 0.000 | -2.61 | 0.016 | -4.07 | 0.001 |
| ATP synthase gamma chain | CAA45150 | 5.E-80 | AW186038 | -4.15 | 0.002 | -2.71 | 0.010 | -5.77 | 0.000 |
| putative leukotriene-A4 hydrolase | AAM91766 | 3.E-50 | AW277270 | -2.08 | 0.025 | -1.67 | 0.019 | -1.88 | 0.004 |
| ATP synthase B' chain | CAA50520 | 4.E-24 | AW471917 | -2.94 | 0.008 | -3.25 | 0.013 | -7.91 | 0.001 |
| granule-bound starch synthase Ib precursor | BAC76613 | 8.E-78 | AW472190 | -4.85 | 0.005 | -5.28 | 0.001 | -8.47 | 0.000 |
| pepsin A | NP_196320 | 4.E-54 | AW568189 | -5.01 | 0.024 | -2.34 | 0.003 | -4.01 | 0.004 |
| plastid ribosomal protein CS17 | CAA77502 | 4.E-32 | AW508645 | -2.47 | 0.008 | -2.16 | 0.005 | -4.58 | 0.000 |
| phosphoglycerate kinase | AAF85975 | 2.E-18 | AW568791 | -3.08 | 0.012 | -2.44 | 0.009 | -6.98 | 0.000 |
| chitinase-like protein | BAC81645 | 1.E-38 | AW508700 | -2.20 | 0.015 | -2.45 | 0.007 | -2.81 | 0.001 |
| unknown | AW508640 | -1.80 | 0.017 | -2.27 | 0.015 | -1.61 | 0.033 | ||
| unknown | AW570244 | -2.03 | 0.004 | -1.97 | 0.001 | -2.81 | 0.001 | ||
| unknown | AW598111 | -2.16 | 0.005 | -1.58 | 0.009 | -2.58 | 0.013 | ||
| unknown | AW508120 | -4.73 | 0.001 | -4.19 | 0.043 | -19.62 | 0.000 | ||
| unknown | AW100867 | -2.40 | 0.007 | -2.00 | 0.001 | -1.65 | 0.016 | ||
| unknown | AI941196 | -1.85 | 0.003 | -2.17 | 0.013 | -2.01 | 0.000 | ||
| unknown | AW164582 | -1.64 | 0.006 | -2.00 | 0.007 | -3.58 | 0.001 | ||
| unknown | AW471578 | -2.03 | 0.025 | -2.13 | 0.003 | -3.51 | 0.000 | ||
| unknown | AW508445 | -3.28 | 0.008 | -2.41 | 0.031 | -1.67 | 0.020 | ||
| unknown | AW507853 | -3.02 | 0.014 | -2.91 | 0.005 | -6.71 | 0.000 | ||
| unknown | AW569116 | -3.17 | 0.002 | -2.02 | 0.008 | -1.47 | 0.038 | ||
| unknown | AW471729 | -2.02 | 0.037 | -3.36 | 0.009 | -2.07 | 0.008 | ||
| unknown | AW568035 | -10.74 | 0.006 | -2.49 | 0.047 | -1.79 | 0.001 | ||
| unknown | AW101065 | -3.07 | 0.008 | -3.62 | 0.006 | -3.41 | 0.033 | ||
| unknown | AW568660 | -6.56 | 0.002 | -2.69 | 0.002 | -3.33 | 0.000 | ||
| Genes induced by TUN and AZC but repressed by PEG | |||||||||
| CDC48-like protein | AAP53974 | 5.E-71 | AW509037 | -1.70 | 0.009 | 10.57 | 0.000 | 5.78 | 0.001 |
| calnexin homolog precursor | BAD81043 | 9.E-78 | AW569128 | -1.76 | 0.044 | 12.82 | 0.000 | 6.09 | 0.000 |
| calnexin homolog precursor | Q39817 | 9.E-59 | AW397007 | -1.67 | 0.005 | 15.69 | 0.001 | 7.16 | 0.000 |
| calnexin homolog precursor | Q39817 | 1.E-82 | AW508066 | -2.00 | 0.015 | 22.76 | 0.000 | 15.03 | 0.000 |
| BiP isoform D | AW569111 | -1.78 | 0.029 | 25.60 | 0.000 | 23.72 | 0.000 | ||
| BiP isoform D | AAK21920 | 3.E-101 | AW509482 | -2.30 | 0.008 | 37.13 | 0.000 | 31.41 | 0.000 |
| BiP | BAD95470 | 1.E-76 | AW471814 | -2.16 | 0.023 | 27.22 | 0.034 | 31.90 | 0.000 |
| BiP | BAD95470 | 5.E-68 | AW507892 | -2.43 | 0.022 | 29.42 | 0.001 | 20.75 | 0.000 |
| Genes repressed by TUN and AZC but induced by PEG | |||||||||
| unknown | AW186103 | 1.74 | 0.003 | -1.77 | 0.049 | -1.72 | 0.005 | ||
* Additional files 1, 2, 3, 4, 5, 6 contain Microarray results that were used to generate Table 1. a Protein annotations obtained from BlastX using cDNA clone sequence against GenBank. b Expected values obtained by BlastX. c Clone accession number in the GenBank. d Fold variation in gene expression converted from means of the log2ratio (treated/control channel) from plants treated with PEG, TUN or AZC and their respective controls. e probability values obtained from the t-test.