Table 2. MVA-BAC genes deleted by GalK recombineering.

Gene	Function of encoded protein	Refs	Left homology arm (bp)a	Right homology arm (bp)a	Mutations in MVAb
C12L	IL-18 binding protein	[41], [42]	13195-13224	13415-13464	6 bp in-frame deletion
A44L	Hydroxysteroid dehydrogenase	[44], [45]	142608-142657	143549-143598	1 amino acid substitution
A46R	Inhibitor of Toll-like receptor signalling	[46]	143941-143990	144699-144748	–
B7R	Chemokine binding protein (SCP-3c)	[36]	156989-157038	157564-157613	15 bp in-frame deletion
B15R	IL-1β binding protein	[51]	161971-162020	162999-163048	–

^aRefers to residue numbering of MVA genome sequence U94848 [5].

^bCoding mutations specific to MVA, i.e. not known in any other strain of Vaccinia virus.

^cSmallpox virus encoded chemokine receptor (SECRET) domain containing protein 3.