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. Author manuscript; available in PMC: 2008 Feb 14.
Published in final edited form as: Biochemistry. 2006 Sep 5;45(35):10682–10689. doi: 10.1021/bi0601151

FIG. 5. Enzyme-linked immuno sorbent assay.

FIG. 5

Protein S was bound to the micro-titre plate through the non-calcium dependent monoclonal antibody HPS56. Peroxidase-conjugated HPS 54, a calcium dependent monoclonal antibody directed against EGF1, was used as a marker. ■ indicates wild-type protein S and ▲ indicates protein S with mutation Asn217Ser. All experiments were performed with 1 mM EDTA in the assay, and the shown concentrations of calcium are the concentrations calculated not to be bound to EDTA.