Table 1. Crystallization conditions for UgpG.

	Crystallization conditions
Crystal	Salt/alcohol	Buffer	Precipitant	Cocrystallization	Drop size/ratio, protein + precipitant (µl)	Crystal dimensions (mm)	Growth time (d)	Temperature (K)	Cryoprotectant	Observations
A1	0.2 M NH4OAc	0.1 M sodium citrate pH 4.6	33% PEG 4K	—	1 + 1	0.02 × 0.02 × 0.08	15	281	25%(v/v) glycerol/mother liquor	—
A2	20% 2-propanol	0.1 M sodium citrate pH 6.0	20% PEG 6K	—	2 + 2	0.03 × 0.03 × 0.10	15	291	25%(v/v) glycerol/mother liquor	Macroseeding
B	0.1 M NH4OAc	0.1 M sodium citrate pH 5.0	31% PEG 4K	—	3 + 3	0.05 × 0.05 × 0.10	21	277	25%(v/v) glycerol/mother liquor	SeMet protein
C	1.6 M (NH4)2SO4	0.1 M Bicine pH 9.0	—	—	3 + 2	0.06 × 0.03 × 0.03	4	293	35%(w/v) sucrose/mother liquor	—
D	1.6 M (NH4)2SO4	0.1 M Bicine pH 9.0	—	—	3 + 2	0.08 × 0.04 × 0.04	7	293	35%(w/v) sucrose/mother liquor	—
E	—	0.1 M sodium citrate pH 5.6	10% PEG MME	+0.5 µl dioxane/drop	5 + 4.5	0.12 × 0.08 × 0.03	2	293	35%(w/v) sucrose/mother liquor	0.1% LM agarose gel, SeMet protein
F	—	0.1 M sodium citrate pH 6.0	15% PEG 4K	10 mM UDP-Glc	2 + 2	0.02 × 0.03 × 0.05	15	293	35%(w/v) sucrose/mother liquor	—
G1	0.1 M NH4OAc	0.1 M sodium citrate pH 4.6	15% PEG MME	5 mM G1P	5 + 2	0.20 × 0.05 × 0.05	15	293	Not needed	30 min soak in 10 mM K2OsO4
G2	0.1 M NH4OAc	0.1 M sodium citrate pH 4.6	13% PEG MME	5 mM G1P	5 + 2	0.10 × 0.04 × 0.04	15	293	Not needed	SeMet protein
G3	0.1 M NH4OAc	0.1 M sodium citrate pH 4.6	15% PEG MME	5 mM G1P	5 + 2	0.20 × 0.05 × 0.05	15	293	Not needed	—