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(a) HIC column chromatography separation of ChiNCTU2 from crude enzyme [empty circles, A 280; filled circles, % (NH4)2SO4]. The column was pre-equilibrated with 1 M ammonium sulfate buffer pH 7.0. (b) Q-column chromatographic separation of the fraction containing ChiNCTU2 [empty circles, A 280; filled circles, % (NH4)2SO4].
