Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
SDS–PAGE of purified BGlu1 fractions from the purification steps for crystallization. Lane 1, Bio-Rad low molecular-weight markers (kDa); lane 2, soluble extract of E. coli cells; lane 3, fusion protein after initial IMAC; lane 4, enterokinase digest; lane 5, BGlu1 after subtractive IMAC; lane 6, BGlu1 after SP-Sepharose, lanes 7 and 8; BGlu1 after the final S200. 5 µg of protein was loaded in all lanes, except lane 7, in which 2.5 µg was loaded.
