Crystals of BGlu1 with and without inhibitor crystallized in an orthorhombic crystal form. (a) Plate-shaped tetragonal crystal of BGlu1 with dimensions 120 × 60 × 16 µm grown in 18%(w/v) PEG 8000, 0.2 M zinc acetate dehydrate and 0.1 M sodium cacodylate pH 6.5 by microbatch screening. (b) BGlu1 protein incubated with DNP2FG crystallized as tetragonal crystals, one with dimensions of 320 × 140 × 20 µm, in a hanging-drop vapour-diffusion plate. The precipitant was 23%(w/v) PEG MME 5000, 0.2 M ammonium sulfate, 0.1 M MES pH 6.7.