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Purification of cytochrome c 4 from the purple photosynthetic bacteria T. roseopersicina. (a) Butyl-Sepharose chromatography. (b) Q-Sepharose chromatography. (c) Phenyl-Sepharose chromatography. (d) Q-Sepharose chromatography. Shaded areas represent cytochrome-containing fractions. For experimental details see text.
