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. 1999 Mar 30;96(7):4119–4124. doi: 10.1073/pnas.96.7.4119

Figure 3.

Figure 3

Effect of the V715M-βAPP mutation on the secretion of total Aβ by HEK293 cells. Stably transfected HEK293 cells overexpressing wild-type βAPP (wt-10 clone), V715M-βAPP (two clones, V715M-6 and V715M-9), or Swedish mutated (Sw)-βAPP were obtained and cultured as described in Experimental Procedures. βAPP expression in cell lysates was revealed with WO2 (A). Total secreted Aβ (B) was immunoprecipitated (with FCA18 antibody), submitted to SDS/PAGE and Western blot analysis (with mAbWO2), and then analyzed as described in Experimental Procedures. Bars in C correspond to densitometric analyses of secreted Aβ (normalized to the amount of endogenous βAPP content) and are the means ± SEM of three to four independent determinations. Mock indicates HEK293 cells stably transfected with the empty pcDNA3 vector. ∗, P < 0.001 (versus wt-10 cells).