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. 1999 Mar 30;96(7):4119–4124. doi: 10.1073/pnas.96.7.4119

Figure 4.

Figure 4

Effect of the V715M-βAPP mutation on the secretion of Aβ40 and Aβ42 and their x-40/42-related products by HEK293 cells. Stably transfected HEK293 cells are as in Fig. 3 and metabolically labeled as described in Experimental Procedures. Expression of βAPP revealed as in Fig. 3 is shown A. Secreted Aβ42/x-42 (B) and Aβ40/x-40 (C) were obtained after sequential immunoprecipitation by means of FCA3542 and FCA3340, respectively, and quantified after radioautography and densitometric analysis. Bars in D and E represent the densitometric analysis, normalized to the amount of endogenous βAPP content, of Aβ42 or Aβ40 (solid bars) and x-42 or x-40 (open bars) and are expressed as the percentage of those obtained with wt-βAPP751-expressing cells taken as 100 (note the distinct scales of ordinates). Values are the means ± SEM of three to eight independent experiments. F illustrates the ratios of Aβ42/Aβ40 (solid bars) and x-42/x-40 (open bars). Mock indicates HEK293 cells stably transfected with the empty vector pcDNA3. ∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001; NS, nonstatistically significant (versus corresponding wt).