Skip to main content
. 1999 Mar 30;96(7):4119–4124. doi: 10.1073/pnas.96.7.4119

Figure 6.

Figure 6

Effect of the V715M-βAPP mutation on the secretion of total sAPP and APPα by HEK293 cells. The indicated transfectants were obtained and metabolically labeled as in Figs. 3 and 4. Cells media were immunoprecipitated with the 207 antibody. Quantification of total sAPP [i.e., APPα + APPβ (A and C)] was performed after SDS/PAGE, Western blot analysis, and direct radioautography as in Fig. 4. APPα (B and D) was analyzed from the same nitrocellulose after hybridization with 10D5C as described in Experimental Procedures. Values are expressed as the percentage of those obtained with wt-βAPP-expressing cells (taken as 100) and are the means ± SEM of three to five independent experiments. ∗, P < 0.001 (versus corresponding wt).