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. 2008 Feb 20;3(2):e1658. doi: 10.1371/journal.pone.0001658

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Salvaing et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Validation of RNAi efficiency by in situ hybridization. Leg imaginal discs from ptc::Gal4>UAS::dsCycG3 third instar larvae were hybridized using CycG as a probe. Expression of the CycG mRNA was specifically reduced in the ptc expression domain along the antero-posterior border (arrow). B. Validation of RNAi efficiency by immuno-labelling. Leg imaginal discs from en::Gal4>UAS::dsCycG3 were immuno-labelled using rabbit anti-CycG antibodies. The expression of CycG was specifically reduced in the en expression domain i.e. in the posterior compartment (arrow), whereas it was ubiquitous in the control (UAS::dsCycG3).