Table 2. PCR, in situ hybridization and immunohistochemical results for paraffin specimens of human control thyroid tissues.
|
B19 status
|
IHC of NF-κB p65
|
||||||
|---|---|---|---|---|---|---|---|
| Case | Age, years | Sex | nPCR | ISH | IHC | Cytoplasmic | Nuclear |
| Normal controls | |||||||
| 1 | 36 | F | + | − | − | − | − |
| 2 | 42 | F | − | − | − | + | − |
| 3 | 67 | F | − | − | − | − | − |
| 4 | 59 | F | + | − | − | ND | ND |
| 5 | 44 | F | + | + | − | ND | ND |
| 6 | 25 | F | − | − | − | − | − |
| 7 | 57 | F | + | + | + | + | − |
| 8 | 49 | F | − | − | − | − | − |
| 9 | 60 | M | − | − | − | − | − |
| 10 | 45 | F | + | − | − | − | − |
| 11 | 58 | F | + | − | − | − | − |
| 12 | 36 | F | − | − | − | − | − |
| 13 | 51 | F | − | − | − | − | − |
| 14 | 40 | F | + | − | − | − | − |
| 15 | 32 | F | − | − | − | − | − |
| 16 | 48 | F | − | − | − | − | − |
| Benign thyroid nodules | |||||||
| 1 | 41 | F | + | + | + | + | − |
| 2 | 52 | F | + | + | + | − | − |
| 3 | 52 | F | + | + | − | + | − |
| 4 | 28 | F | + | − | − | − | − |
| 5 | 56 | M | + | − | − | − | − |
| 6 | 41 | M | + | − | − | − | − |
| 7 | 22 | F | + | − | − | − | − |
| 8 | 59 | M | + | − | − | + | − |
| 9 | 21 | F | + | + | − | − | − |
| 10 | 58 | F | + | + | + | − | − |
| 11 | 54 | F | − | − | − | − | − |
| 12 | 43 | F | − | − | − | ND | ND |
| 13 | 39 | F | − | − | − | − | − |
| 14 | 38 | F | + | + | − | − | − |
| 15 | 42 | F | + | + | + | + | − |
| 16 | 34 | F | + | − | − | − | − |
| 17 | 51 | F | + | − | − | + | − |
| 18 | 68 | F | + | + | + | + | − |
| 19 | 41 | F | + | + | − | − | − |
| 20 | 25 | F | + | + | + | + | − |
| 21 | 28 | F | + | + | − | + | + |
| 22 | 40 | F | + | + | − | − | − |
| 23 | 53 | F | + | + | + | + | + |
| 24 | 50 | F | + | + | − | + | − |
| 25 | 35 | F | + | + | − | + | − |
| 26 | 51 | M | − | − | − | + | − |
| 27 | 44 | M | + | + | − | + | + |
| 28 | 62 | M | + | + | + | + | − |
| 29 | 21 | F | − | − | − | − | − |
| 30 | 39 | F | + | + | − | ND | ND |
| 31 | 54 | F | + | + | − | + | − |
| 32 | 57 | F | + | − | − | − | − |
| 33 | 54 | F | + | + | − | + | − |
| 34 | 41 | F | + | − | − | − | − |
| 35 | 46 | F | + | + | − | + | − |
| 36 | 48 | F | + | + | − | − | − |
| 37 | 51 | F | + | − | − | − | − |
| 38 | 32 | F | + | + | − | − | − |
| 39 | 63 | F | + | + | − | + | − |
| Medullary thyroid carcinoma | |||||||
| 1 | 33 | F | − | − | − | + | + |
| 2 | 68 | F | − | − | − | − | − |
| 3 | 68 | F | + | + | − | + | + |
| 4 | 55 | M | + | − | − | + | + |
| 5 | 30 | F | + | − | − | − | − |
| 6 | 39 | F | − | − | − | − | − |
| 7 | 58 | M | − | − | − | + | + |
| 8 | 46 | F | + | − | − | − | − |
| 9 | 32 | F | + | − | − | − | − |
| Follicular thyroid carcinoma | |||||||
| 1 | 37 | F | + | + | − | − | − |
| 2 | 72 | F | + | + | − | + | − |
| 3 | 35 | M | + | − | − | − | − |
| 4 | 57 | F | + | − | − | − | − |
| 5 | 56 | F | + | + | − | − | − |
| 6 | 33 | F | + | + | − | − | − |
| 7 | 64 | F | + | + | − | − | − |
| 8 | 57 | F | + | + | − | − | − |
| 9 | 41 | F | + | + | − | − | − |
| 10 | 70 | F | + | − | − | − | − |
IHC=immunohistochemistry; ISH=in situ hybridisation; ND =not done as the blocks were used up; NF-κB=nuclear factor kappa B; nPCR=nested PCR.