Figure 7.

Changes in protein carbonyl content after MCA occlusion in Tg and WT mice. The DNP-derivatized protein samples prepared from brains subjected to MCA occlusion were separated on a 15% SDS/PAGE followed by Western blotting with primary antibody, specific to the DNP moiety of the proteins. In sham control animals, only a small amount of DNP moieties was detected, and no difference was detected between the two groups. One hour after ischemia, more DNP moieties were detected in WT mice than in Tg mice (I; ischemia, S; sham).