Figure 1.

Characterization of PKA subunit gene expression in parental and transfected cells. A, Expression of PKA subunit proteins. Cell extracts were prepared as described earlier and 10 μg protein subjected to Western blot analysis (8). B, Cell proliferation in monolayer culture. Cells were plated at 2 × 10³ cells in 60-mm culture dishes, harvested at the indicated times by trypsinization, and counted using a Z1 Coulter Counter (Beckman Coulter). Results are expressed as the mean cell number per milliliter ± sd.