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. 2007 Dec 4;93(2):565–571. doi: 10.1210/jc.2007-1902

Figure 2.

Figure 2

Differential expression of PKA subunits affected expression of E2F genes. A, Western blot analysis of parental and RIα- and RIIβ-transfected cells. Cell lysates were prepared, subjected to SDS-PAGE, and then transferred to nitrocellulose membranes and immunoblotted. B, Differential expression of PKA subunits affected the levels of E2F1 and E2F4. Cell extract was prepared from parental and transfected cells and analyzed by Western blotting. C, Down-regulation of expression of E2Fs affected the growth of parental cells. Cells were transfected with E2F siRNA, and after 24 h, they were harvested and counted, and their extract was subjected to Western blot analysis with E2F-specific antibodies. Results are expressed as the mean cell number per milliliter ± sd. *, Statistical significance.