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. Author manuscript; available in PMC: 2008 Feb 14.
Published in final edited form as: J Biol Chem. 2006 May 30;281(31):22062–22072. doi: 10.1074/jbc.M601155200

FIGURE 3. Ectopic DNMT1 and DNMT3B, but not DNMT3A, inhibit rDNA promoter activity irrespective of its methylation status.

FIGURE 3

A, HeLa cells were transiently transfected with DNMT1, DNMT3A, or DNMT3B expression vectors. Whole cell extracts from these cells were subjected to Western blot analysis with anti-FLAG M2 antibody. B, HeLa cells were transfected with either mock methylated pHrD-IRES-Luc (Unmethylated) or M.HhaI-methylated pHrD-IRES-Luc (Methylated) along with 4μg of the empty vector or pcDNMT1, pcDNMT3A, or pcDNMT3B. After 48 h firefly luciferase activity was measured in the cell extracts. Results are represented as rDNA promoter activity upon overexpression of different DNMTs with empty vector-transfected pHrD-IRES-Luc activity as 100.