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. Author manuscript; available in PMC: 2008 Feb 14.
Published in final edited form as: J Biol Chem. 2006 Aug 22;281(43):32909–32921. doi: 10.1074/jbc.M602454200

FIGURE 2. Removal of a single activation domain of Hsf1 reveals promoter-specific defects in heat shock induction of HSPs in the absence of msn2/4. A.

FIGURE 2

and B, total RNA was isolated from six different strains that were either maintained at constitutive conditions (30 °C) or heat shocked at 37 °C for 15 min. A representative Northern blot is shown for the transcripts HSP104, HSP12, HSP26, and ACT1 (loading control). The bar graph below illustrates heat induction, which was calculated as the -fold change of the normalized transcript between heat shock conditions versus constitutive conditions. The asterisks indicate significant differences between wild-type and the condition tested using Student’s t test: **, p < 0.01; ***, p < 0.001.