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Steady-state kinetic parameters for putrescine of wild-type PuORh and of the mutants Glu324Ala and Glu324Leu at pH 9.0.
| K m (μM) | k cat (s−1) | k cat/K m (s−1 mM−1) | |
|---|---|---|---|
| PuORh WT | 3.5 ± 0.6 | 20.7 ± 1.1 | 5900 |
| PuORh Glu324Ala | 110,000 ± 20,000 | 6.1 ± 0.4 | 0.05 |
| PuORh Glu324Leu | 110,000 ± 20,000 | 3.2 ± 0.2 | 0.03 |
