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STX suppresses the sensitivity of Ig to calcium changes even in the presence of external Na+. The external solutions were 100Ca 100Na and 10Ca 100Na. Channels were activated at +40 mV and deactivated at −90 mV. About half of the inactivation gates had been removed by internal perfusion with Pronase. As expected, the effect of Pronase treatment on the gating current tail after the short pulse illustrated was negligible. Temperature: 8°C.
