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ZmWee1 inhibits CDK activity in vitro. GST-ZmWee1 fusion protein from E. coli was purified by affinity chromatography with glutathione agarose and added to a histone H1 phosphorylation reaction containing p13suc1-adsorbed CDK from immature maize ears (A) or total protein extract from immature ears (B). The autoradiograph indicates the degree of 32P-labeling of histone H1. ZmWee1 inhibited histone H1 kinase activity of the p13suc1-adsorbed CDK as well as that in immature ear extract.
