Seed development in wild-type and mutant Arabidopsis plants. Siliques and seeds shown in panels A–G were harvested 9 days after self-pollination. Those in panels H–I were harvested 5 days after self-pollination. Seeds in F–I were cleared and photographed by using Nomarski optics as described in Methods. (A) Portion of a wild-type silique. (B) Portion of an f644 heterozygous silique. (C) Portion of an f644 homozygous silique. (D and F) Seeds with wild-type phenotype from f644 heterozygous silique in B. (E and G) Seeds with mutant phenotype from f644 heterozygous silique in B. Arrows in Inset point to nuclei. (H) Wild-type seed. (I) Homozygous f644 seed. AE, abnormal embryo; EM, embryo; EN, endosperm; m, seed with a mutant phenotype; wt, seed with a wild-type phenotype; SC, seed coat. [Bar = 0.5 mm (A–C), 0.1 mm (D–I), and 0.04 mm (Inset in G).]