Table 2.
Products formed from the reaction of cslbLOX and of cslbLOXH608V with either LA or TL
| Mutants | Products obtained with LA
|
Products obtained with TL
|
|
|---|---|---|---|
| Positional isomers 13-HODE:9HODE and major enantiomer | Amount of TL oxygenation and molar ratios of oxygenation products, TL-3HODE : TL-2HODE : TL-1HODE | Positional isomers 13-HOME:9-HODE and major enantiomer | |
| cslbLOX | 84 : 16 | 100% | 90 : 10 |
| S : S | 44 : 30 : 36 | S : rac | |
| H608V | 5 : 95 | 42% | 65 : 35 |
| rac : S | 2 : 26 : 72 | S : rac | |
| H608M | 21 : 79 | 71% | 83 : 17 |
| S : S | 25 : 34 : 41 | S : rac | |
| T607I | 45 : 55 | 74% | 85 : 15 |
| S : S | 19 : 29 : 52 | S : rac | |
| T607IH608V | 11 : 89 | 88% | 72 : 28 |
| s : S | 18 : 30 : 52 | S : rac | |
| T607IH608M | 18 : 82 | 90% | 80 : 20 |
| S : S | 18 : 33 : 49 | S : rac | |
| R758LH608V | 60 : 40 | 20% | 50 : 50 |
| rac : rac | 5 : 29 : 66 | rac : rac | |
| R758LT607IH608V | 52 : 48 | 17% | 50 : 50 |
| rac : rac | 2 : 31 : 67 | rac : rac | |
S > 80% S-enantiomer; s = 70–80% S-enantiomer; rac = 50–70% S-enantiomer, racemic. Oxygenated fatty acid derivatives were isolated by RP-HPLC (shown for TL in Fig. 4). Positional isomers of hydroxylinoleic acid (HODE) were given as molar ratios determined by SP-HPLC (Fig. 3). Optical isomers were determined by CP-HPLC (Fig. 3 Insets).