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The incorporation of 14C from d-[U-14C]mannose into 14C-AsA by Arabidopsis leaves is less in vtc1-1 (○) than wt (▴). Excised leaves were labeled with d-[U-14C]mannose. The AsA-containing fraction from soluble extracts of these leaves was fractionated by HPLC. The AsA peak was collected in 30 fractions, which were individually assayed for 14C. The results are expressed as a percentage of total 14C incorporated into AsA. Three replicate samples were processed for each genotype.
