Table 1.
16S variable region range definitions.
| Variable region | E. coli 16S rDNA range | 5' primer | 3' primer | ||
| start | end | length | |||
| V1 | 8 | 120 | 113 | 5'-AGAGTTTGATCMTGGCTCAG | 5'-TTACTCACCCGTICGCCRCT |
| V2 | 101 | 361 | 261 | 5'-AGYGGCGIACGGGTGAGTAA | 5'-CYIACTGCTGCCTCCCGTAG |
| V3 | 338 | 534 | 197 | 5'-ACTCCTACGGGAGGCAGCAG | 5'-ATTACCGCGGCTGCTGG |
| V4 | 519 | 806 | 288 | 5'-TGCCAGCAGCCGCGGTAA | 5'-GGACTACARGGTATCTAAT |
| V5 | 787 | 926 | 140 | 5'-ATTAGATACCYTGTAGTCC | 5'-CCGTCAATTCMTTTGAGTTT |
| V6 | 907 | 1073 | 167 | 5'-AAACTCAAAKGAATTGACGG | 5'-ACGAGCTGACGACARCCATG |
| V7 & VV8 | 1054 | 1406 | 353 | 5'-CATGGYTGTCGTCAGCTCGT | 5'-ACGGGCGGTGTGTAC |
| V9 | 1392 | 1507 | 116 | 5'-GTACACACCGCCCGT | 5'-TACCTTGTTACGACTT |
Regions were chosen to be mostly non-overlapping, each containing one or two variable regions. Coordinates are given relative to the 1542 bp E. coli K12 16S rDNA sequence.