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. 1998 Apr 14;95(8):4146–4151. doi: 10.1073/pnas.95.8.4146

Figure 3.

Figure 3

Comparison of the time courses of phosphorylation of peptide PC9 by the following MIHCK catalytic domains: ▪, phosphorylated wt (12 μg/ml); ▴, dephosphorylated wt (4 μg/ml); ▿, rephosphorylated wt (3.5 μg/ml); ○, the S627A mutant (42 μg/ml). Kinase was dephosphorylated and rephosphorylated as described (17). The peptide concentration was 1 mM and ATP was 2.5 mM. Activity is reported as mol of peptide phosphorylated per mol of catalytic domain. See text for details.