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. 2003 Sep;77(18):9872–9884. doi: 10.1128/JVI.77.18.9872-9884.2003

FIG. 2.

FIG. 2.

ICP27 transactivates the gC gene, in combination with ICP4 and ICP0. (A). Induction of gC protein expression. Vero cells were transfected with pUC19 only (lane 3) or with pgC (lanes 4 to 7). Transfections also included pSG1, which encodes ICP4 and ICP0 (lane 5); pC27, which encodes ICP27 (lane 6); or pSG1 plus pC27 (lane 7). After 2 days, cell lysates were prepared and equal amounts were subjected to immunoblotting using a monoclonal antibody specific for gC. Lane 1 shows protein size standards; lane 2 contains protein extract from HSV-1-infected Vero cells, harvested at 6 hpi. (B) Induction of gC mRNA expression. Vero cells were transfected with the plasmids indicated, as in panel A. RNA was prepared at 2 days posttransfection, and equal amounts were subjected to Northern analysis using a gC gene-specific probe. Lane 1 contains RNA from HSV-1-infected Vero cells. (C) ICP8 gene expression is not affected by ICP27. Vero cells were transfected as in panel A, except that plasmid pSG18, which encodes ICP8, replaced pgC. Protein analysis was carried out as in panel A, except the blot was probed with an ICP8-specific monoclonal antibody.