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. 2003 Sep;77(18):9872–9884. doi: 10.1128/JVI.77.18.9872-9884.2003

FIG. 3.

FIG. 3.

ICP27 and the tet-TA can cooperate to induce gC gene expression. (A) Induction of gC protein expression. Vero cells were transfected with pUC19 only (lane 2) or with a gC-encoding plasmid (lanes 3 to 8), either pgC or pgCXTO (top and bottom panels, respectively). Transfections also included pSG1, which encodes ICP4 and ICP0 (lane 4); pBH27, which encodes ICP27 (lane 5); pSG1 plus pBH27 (lane 6); pUHD15-1, which encodes tet-TA (lane 7); or pUHD15-1 plus pBH27 (lane 8). Protein analysis was carried out as described for Fig. 2. Lane 1 contains protein extract from HSV-1-infected cells. (B) Induction of gC mRNA expression. Vero cells were transfected with the plasmids indicated, as in panel A. RNA was prepared at 2 days posttransfection, and equal amounts were subjected to Northern analysis using a gC gene-specific probe.