FIG. 6.

Isolation of HSV-1 gC-negative mutants. (A) Schematic representation of the gC gene modification in the virus mutants d44 and d44/27. The gC gene open reading frame was replaced with that of the E. coli lacZ gene, as shown. The mutant d44 was derived from WT HSV-1, whereas d44/27 was derived from the ICP27 deletion mutant d27-1. (B) The d44 and d44/27 mutants express β-galactosidase but not gC. V27 cells were mock infected or infected with the viruses indicated. Proteins were harvested at 6 hpi and subjected to immunoblot analysis using monoclonal antibodies specific for ICP8, gC, and β-galactosidase (top, middle, and bottom panels, respectively).