FIG. 1.

Structure of HCoV vector RNA. (A) The structural relationship and sizes of the RNAs and ORFs of HCoV 229E and vec-CLG vector is shown. TAS elements encoded by HCoV 229E or vec-CLG vector are shown as arrows. TAS elements that mediate the synthesis of HCoV 229E subgenomic mRNAs encoding S, M, and N proteins have been inserted into vec-CLG RNA and are indicated by black arrows. (B) TAS element-containing RNA sequences of vec-CLG RNA are shown in relation to ORFs encoding the replicase gene, CAT gene, LUC gene, and GFP gene, respectively. Core sequences are depicted in boxes; stop and start codons of the respective ORFs are underlined. Nucleotides that match the HCoV 229E sequence are shown as capital letters.