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Sequencing analysis of subgenomic mRNAs isolated from vec-CLG-transfected cells. Poly(A)-containing RNA was isolated from vec-CLG RNA and N protein mRNA transfected cells. The TAS regions of vec-CLG subgenomic mRNAs encoding CAT (A), LUC (B), and GFP (C) were analyzed by RT-PCR amplification and cycle sequencing. The sequences corresponding to HCoV leader (L), core sequences of the TAS elements (in boxes), and the reporter gene start codons (underlined) are shown.
