Figure 8.

Morphological alterations of P. patens grown under Pi-deficient conditions. A, P. patens grown on +Pi or −Pi medium for 28 d. B, Magnification of +Pi petri dish shown in A. C, Magnification of −Pi petri dish shown in A. D and E, Protonemata at the edge of colonies grown on +Pi (D) and −Pi (E) medium. F and G, Growth differences of P. patens colonies from protonemata distributed onto petri dishes containing +Pi or −Pi medium and grown for 14 d. The diameter of individual colonies (n = 20; F) and the plant biomass in each petri dish (n = 5; G) were measured. H and I, Gametophores grown in +Pi and −Pi medium for 90 d with measurements of the length of the leafy shoot and rhizoid (n = 8; I). Bars = 1 cm (B and C) and 60 μm (D and E).