Figure 1.

Cytochrome b₆f and Rubisco content in leaf and epidermis of wild-type and transgenic tobacco plants determined by immunoblotting. Total protein extracts of leaf discs and epidermal fragments are compared for content of cytochrome f (cyt f in the image) and the large subunit and SSU of Rubisco (Lsu and Ssu in the image, respectively). Plants were grown under LL or ML intensity for comparison of wild-type with anti-b/f and anti-SSU leaves, respectively. Three different anti-b/f plants, labeled a-bf1, a-bf2, and a-bf3, and having CO₂ assimilation rates that were 43%, 36%, and 17% of wild-type values, respectively, are shown as representatives of anti-b/f plants with low photosynthetic rates. For whole-leaf samples, gel lanes were loaded on an equal-leaf-area basis, and samples from ML-grown plants were diluted 5-fold relative to samples from LL-grown plants. Equal total protein amounts (20 μg) were loaded when comparing epidermal samples. wt, Wild-type plant; a-Ssu, anti-SSU plant.