Figure 2.

The gene and protein of peroxidase 1 from C. roseus (CrPrx1). A, Schematic representation of the gene (P, promoter; Int, intron; Ex, exon; UTR, untranslated region). B, Nucleotide and deduced amino acid sequence. The coding region is shown in uppercase letters. The signal peptide and the putative C-terminal propeptide are represented in italics. Sequences highlighted correspond to the peroxidase active site signature (positions 38–49) and to the peroxidase proximal heme-ligand signature (positions 168–178). The single amino acids highlighted are the eight conserved Cys, which allow the formation of four disulfide bridges. Sequences underlined in black match perfectly the sequences determined for the nine sequenced peptides (Table I). Sequences underlined in gray correspond to putative glycosylation sites. Boxes, aataaa polyadenylation signals. Arrowheads, Different positions of the poly(A) tail determined by RACE for seedlings. Arrows, Different positions of the poly(A) tail in two clones isolated from the cDNA library prepared from the first pair of leaves. Accession numbers are: AM236087 (mRNA), AM236088 (promoter region), and AM236089 (intron I).