Table I.

Agrobacterium T-DNA binary vectors

Vector Series Name	Vector ori/Incompatibility Group	Important Featuresa	Gateway Compatable	Bacterial Selection Markerb	Plant Selection Markerb	Reference
pBIN	IncPα	mcs with blue/white selection	No	Kan	Kan	Bevan (1984)
pGA	IncPα	cos site ColE1 ori	No	Kan	Kan	An et al. (1985); An (1987)
SEV	IncPα	Reconstitutes a missing T-DNA border; not a binary vector	No	Kan	Kan/Nos	Fraley et al. (1985)
pEND4K	IncPα	cos site, mcs with blue/white selection	No	Kan/Tet	Kan	Klee et al. (1985)
pBI	IncPα	Promoterless gusA gene for promoter studies	No	Kan	Kan	Jefferson et al. (1987)
pCIB10	IncPα	Chimeric antibiotic-resistance gene	No	Kan	Chimeric Kan/Hyg	Rothstein et al. (1987)
pMRK63	pRi	pRi-based vector (borders from pRi)	No	Amp/Kan	Kan	Vilaine and Casse-Delbart (1987)
pGPTV	IncPα	Promoterless gusA gene for promoter studies	No	Kan	Kan/Hyg/Bar/Bleo/Dhfr	Becker (1990)
pCGN1547	pRi + ColE1	ColE1 ori for high copy no. in E. coli mcs with blue/white selection	No	Gent	Kan	McBride and Summerfelt (1990)
pART	IncPα + ColE1	ColE1 ori for high copy no. in E. coli promoter/polyA expression cassette	No	Spec	Kan	Gleave (1992)
pGKB5	pRiA4	Promoterless gusA gene for promoter studies	No	Kan	Kan/Bar	Bouchez et al. (1993)
pMJD80 pMJD81	IncPα	Ω, untranslated leader	No	Kan	Kan	Day et al. (1994)
pPZP	pVS1	Small, stable, mcs with blue/white selection	No	Spec/Chl	Kan/Gent	Hajdukiewicz et al. (1994)
pBINPLUS	IncPα	Selectable marker near LB ColE1 ori	No	Kan	Kan	van Engelen et al. (1995)
pRT100 pRT-Ω/Not/Asc	IncPα	Rare-cutting sites (NotI, AscI)	No	Kan	Kan/Hyg/Bar/Dhfr	Uberlacker and Werr (1996)
BIBAC	pRi	T-DNA binary vector designed to transfer large DNA fragments	No	Kan	Hyg	Hamilton (1997)
pCB series	IncPα	Mini binary vectors small backbone, not self-mobilizable	No	Kan	Bar	Xiang et al. (1999)
pGreen	IncW	ColE1 ori mcs with blue/white selection	No	Kan	Kan/Hyg/Sul/Bar	Hellens et al. (2000a)
pPZP-RCS2	pVS1	Multiple rare-cutting sites for cassette insertion. Uses pPZP200 as backbone	No	Spec	Kan/Gent	Goderis et al. (2002)
GATEWAY destination vector	pVS1	ColE1 ori. Uses pPZP200 as backbone	Yes	Spec	Kan/Hyg/Bar	Karimi et al. (2002)
pMDC	pVS1	Based on pCAMBIA (except pMDC7, from PER8). Facilitates protein tagging	Yes	Kan; Spec for pMDC7	Kan/Hyg/Bar	Curtis and Grossniklaus (2003)
pRCS2	pVS1	Contains rare-cutting sites	No	Spec	Kan/Hyg/Bar	Chung et al. (2005)
pRCS2-ocs	pVS1	Cloning of multiple genes	No	Spec	Kan/Hyg/Bar	Tzfira et al. (2005)
pEarleyGate	pVS1	Based on pCAMBIA. Facilitates protein tagging	Yes	Kan	Bar	Earley et al. (2006)
pGWTAC pMDC99	pRiA4	Multi-Round Gateway for cloning multiple genes	Yes	Kan	Hyg	Chen et al. (2006)
pORE	IncPα	Based on pCB301 ColE1 ori FRT sites. Promoterless gusA or gfp gene for promoter studies	No	Kan	Kan/Pat	Coutu et al. (2007)
pSITE	pVS1	Fluorescence protein fusion. Based on pRCS2	Yes	Spec	Kan	Chakrabarty et al. (2007)
pMSP	IncPα	Super-promoter to drive expression of goi	No	Kan	Kan/Hyg/Bar	Lee et al. (2007)
pCAMBIA	pVS1	Multiple vectors for cloning, expression, and tagging	No	Kan/Chl	Kan/Hyg/Bar	http://www.cambia.org/daisy/cambia/materials/vectors
pGD	PVS1	Derived from pCAMBIA1301. Multiple vectors for tagging proteins with DsRed2 or GFP	No	Kan	Hyg	Goodin et al. (2002)

^acos, Bacteriophage λ cohesive ends; mcs, multiple cloning site; ori, vegetative origin of replication; Ω, tobacco mosaic virus translational enhancer.

^bAmp, Ampicillin; Bar, resistance to phosphinothricin; Bleo, bleomycin; Chl, chloramphenicol; Dhfr, dihydrofolate reductase; Gent, gentamicin; Hyg, hygromycin; Kan, kanamycin, Nos, nopaline synthase; Pat, resistance to phosphinothricin; Spec, spectinomycin; Sul, sulfonylurea; Tet, tetracycline.