Reduction in PA P correlates with increased Pi and phytase enzyme activity in mature CAPPA seed samples. A, Individual mature seed samples were subjected to three analyses: PA P, Pi P, and phytase enzyme activity. The sum of Pi P and PA P is represented for each T3 seed sample. Samples 1 through 10 were derived from 14H (CAPPA/CAPPA), while samples 11 to 15 were derived from 14N (CAPPA/−) and were segregating for the transgene event. B, Phytase enzyme activity was determined for individual seed samples carried out at 55°C.