Figure 3.

Isoform specificity of RCN1 function in root tip organization. Median longitudinal sections of propidium iodide-stained 4-dpg root tips were captured using confocal laser microscopy, revealing normal meristem organization in wild-type (Ws) roots and highlighting the disorganization of columella and initial cells in rcn1 root tips. Examples of moderate (rcn1 moderate) and severe (rcn1 severe) rcn1 disorganization phenotypes are shown. The YFP-RCN1 fusion restores wild-type morphology in rcn1 plants carrying the RYR construct, whereas the YFP-PP2AA3 fusions carried in RYA and AYA transformants fail to fully rescue the rcn1 phenotype. Brackets indicate the region of the QC; asterisks indicate cells representative of clearly defined columellar cell files. Scale bars, 25 μm.