Table III.
Comparisons in the relative fold changes in peak areas for selected identified compounds in two independent elicitation experiments
Metabolites are listed if the changes in their levels showed a similar response, at a significant value of P < 0.05, in the two experiments (large-scale experiments in which YE and MeJA elicitations were applied to consecutive passages of the culture and cultures sampled at 21 time points postelicitation, and the additional smaller experiment in which YE and MeJA elicitation were carried out in parallel on a single passage culture). Values represent the fold change in peak area at the average of the 3-, 12-, and 24-h time points (values >1.0 represent increased levels in elicited samples, values <1.0 represent decreased levels due to elicitation; empty cells, not significant in at least one of the two Student's t tests).
| Compound | YE
|
MeJA
|
||
|---|---|---|---|---|
| YE | Parallel | MeJA | Parallel | |
| p-Hydroxybenzaldehyde | 5.5 | 9.3 | – | – |
| genistin | 3.5 | 3.8 | – | – |
| 5,3′-Dimethoxyluteolin glucoside | 1.7 | 1.8 | – | – |
| Genistein glucoside malonate | – | – | −0.7 | – |
| Hispidol glucoside malonate | 4.7 | 6.2 | – | – |
| 5,3′-Dimethoxyluteolin glucoside malonate | 1.4 | 1.4 | – | – |
| Ononin | – | – | −0.5 | −0.6 |
| Afrormosin glucoside | – | – | −0.8 | −0.8 |
| 2′-Hydroxyformononetin glucoside malonate | – | – | −0.5 | – |
| Afrormosin glucoside malonate | – | – | −0.8 | – |
| Formononetin glucoside malonate | – | – | −0.6 | – |
| Naringenin | 8.3 | 10.1 | – | – |
| Isoliquiritigenin | 2.1 | 4.5 | – | – |
| Alfalone | 13 | 14.6 | – | – |
| Formononetin | 5.3 | 6.2 | – | – |
| Afrormosin | 3.9 | 5.1 | 3.2 | 2.8 |
| Medicarpin | 8 | 9.6 | 5 | 5.2 |
| Biochanin-A | 2.7 | 4.1 | 3 | – |
| Irisolidone | 3 | – | – | – |