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. 2008 Feb;146(2):418–430. doi: 10.1104/pp.107.110809

Figure 1.

Figure 1.

Affinity purification of plant AAH candidates. Purification of C terminally StrepII-tagged AtAAH, AtAAH-2, and GmAAH from leaf extracts of N. benthamiana with StrepTactin affinity chromatography. Lanes 1 to 4, Purification of AtAAH visualized by Coomassie Blue staining, 12 μL of protein solution loaded per lane. Lane 1, Extract of soluble proteins; lane 2, proteins not bound after incubation with StrepTactin affinity matrix; lane 3, protein in fifth wash supernatant; lane 4, pool of eluted protein; lane 5 (12 μL loaded), pool of eluted AtAAH visualized by western blotting and StrepTactin-alkaline phosphatase conjugate detection; lanes 6 to 8 (4 μL loaded), purity of AtAAH, GmAAH, and AtAAH-2 visualized by silver staining.