Figure 2.

Gene expression and protein appearance for Trx h and NTR isoforms in barley embryos during germination (0–144 h). A, RT-PCR analysis of HvTrxh1, HvTrxh2, HvNTR1, and HvNTR2. One representative gel is shown from three independent replicates. Relative band intensities were normalized to the 18S rRNA band intensity (100%). Each histogram represents the mean ± sd obtained from three independent RT-PCR reactions. B, Western blot of embryo protein extracts using antibodies against Trx h and NTR.