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. 2008 Feb;146(2):789–799. doi: 10.1104/pp.107.113639

Figure 4.

Figure 4.

Expression, purification, and chemical properties of NTR. A, SDS-PAGE of NTR overexpression in E. coli, purification, and thrombin cleavage. Total protein from E. coli harboring pET15b-HvNTR1 (lanes 1 and 2) and pET15b-HvNTR2 (lanes 4 and 5), respectively, before (lanes 1 and 4) and 3.5 h after (lanes 2 and 5) induction with IPTG; purified His-HvNTR1 and His-HvNTR2, respectively (lanes 3 and 6); and HvNTR2 after thrombin cleavage to remove the His-tag (lane 7). B, Absorption spectrum of recombinant HvNTR2. C, MALDI MS spectrum of intact His-HvNTR2 and after cleavage with thrombin.