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GC-MS analysis of the product of the enzyme assay catalyzed by recombinant OstIAMT1 protein with IAA as the substrate. A, GC chromatogram of the hexane extract of an OsIAMT1 enzyme assay with IAA and the mass fragmentation spectrum of the methylated product. B, GC chromatogram of a MeIAA authentic standard and its mass fragmentation spectrum.
