FIG. 1.

Evaluation of HFRS DNA vaccine in ANDV-hamster lethal-disease model. Hamsters were vaccinated with pWRG/HTN-M(x) or the negative control plasmid pWRG7077 and then challenged with ANDV. For animals that succumbed, the day of death is shown in parentheses. Animals that survived the first challenge were rechallenged with ANDV. Sera drawn on the day of challenge (pre-challenge), 4 to 6 weeks after challenge (post-challenge), and 28 to 48 days after a second challenge (post-rechallenge) were tested for HTNV- and ANDV-specific NAbs by PRNT. The bars represent PRNT titers. Hamster identification numbers (ID#) 68 to 91 and 501 to 523 represent two independent experiments. N, not done.